Adaptation of Newcastle Disease Virus (NDV) on Vero Cell Line
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چکیده
منابع مشابه
Enumeration Techniques of Newcastle Disease Virus (NDV) for Oncolytic Virotherapy
The present work was carried out to evaluate different quantitation techniques when Newcastle disease virus was intended to use as an oncolytic agent. The R 2 B Mukteshwar strain of NDV was procured as lyophilized vaccine. Then application were carried out like haemagglutination test, tissue culture infective dose-50 (TCID50), plaque forming unit (PFU) calculation and real time PCR to enumerate...
متن کاملConstruction of Recombinant Bacmid DNA Encoding Newcastle Disease Virus (NDV) Fusion Protein Gene
Background and Aims: Newcastle disease virus (NDV) is one of the major pathogen in poultry. Vaccination is intended to control the disease as an effective solution nevertheless this virus is a growing threat to the poultry industry. F gene open reading frame (ORF) from NDV is 1650 bp, encoding a protein of 553 amino acids that can induce protective immunity alone. The F glycoprotein on the surf...
متن کاملA recombinant Newcastle disease virus (NDV) expressing VP2 protein of infectious bursal disease virus (IBDV) protects against NDV and IBDV.
Infectious bursal disease virus (IBDV) causes a highly immunosuppressive disease in chickens. Currently available, live IBDV vaccines can lead to generation of variant viruses. We have developed an alternative vaccine that will not create variant IBDV. By using the reverse genetics approach, we devised a recombinant Newcastle disease virus (NDV) vector from a commonly used vaccine strain LaSota...
متن کاملoncolytic effect of newcastle disease virus af2240 strain on the mcf-7 breast cancer cell line
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متن کاملEvaluation of immunogenic potential of 75kDa and 56kDa proteins of newcastle disease virus (NDV).
The R2B strain of virus of new castle disease virus (NDV) was propagated in 9-11 day old embryonated chicken eggs via allantoic cavity route and after seven serial passages virus was purified from allantoic fluid. Purified virus was analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis which yielded six major polypeptides ranging from 38-200 kDa. Protein fractions, corresponding...
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ژورنال
عنوان ژورنال: International Journal of Poultry Science
سال: 2004
ISSN: 1682-8356
DOI: 10.3923/ijps.2004.153.156